Distinct contributions of DNA methylation and histone acetylation to the genomic occupancy of transcription components
Epigenetic modifications on chromatin play very important roles in regulating gene expression. Although chromatin states are typically dominated by multilayered development, how explicit individual pathways contribute to gene expression stays poorly understood. As an example, DNA methylation is known to handle transcription subject binding however along with recruit methyl-CpG binding proteins that impact chromatin development by the train of histone deacetylase complexes (HDACs).
Every of these mechanisms can most likely impact gene expression, nevertheless the importance of each, and whether or not or not these actions are built-in to appreciate acceptable gene regulation, stays largely unknown.
To take care of this very important question, we measured gene expression, chromatin accessibility, and transcription subject occupancy in wild-type or DNA methylation-deficient mouse embryonic stem cells following HDAC inhibition.
We observe widespread will enhance in chromatin accessibility at retrotransposons when HDACs are inhibited, and that’s magnified when cells moreover lack DNA methylation. A subset of these elements has elevated binding of the YY1 and GABPA transcription elements and elevated expression.
The pronounced additive affect of HDAC inhibition in DNA methylation-deficient cells demonstrates that DNA methylation and histone deacetylation act largely independently to suppress transcription subject binding and gene expression.
Identification of the DNA Replication Regulator MCM Superior Expression and Prognostic Significance in Hepatic Carcinoma
Background: The microliposome maintenance (MCM) superior, MCM2-7, is revealed to be involved in a lot of cell processes and performs a key place inside the development and growth of human cancers. Nonetheless, the MCM superior stays poorly elaborated in hepatic carcinoma (HCC).
Methods: Throughout the analysis, we found the mRNA and protein diploma by bioinformatics. We moreover explored the prognostic value, genetic alteration, interaction neighborhood, and sensible enrichment of MCM2-7. The MCM expression and correlation amongst these MCMs in HCC cell strains have been acknowledged by western blot.
Outcomes: MCM2-7 was significantly elevated in HCC tissues as compared with common liver tissues. The extreme diploma of MCM2-7 had a constructive correlation with poor prognosis. Nonetheless, MCM2-7 alterations weren’t correlated with poor OS. MCMs have been every elevated in HCC cell strains as compared with the traditional hepatocyte cell line. Furthermore, the constructive correlation was found amongst MCMs in HCC cell strains.
Conclusions: The MCM superior was elevated in HCC tissues and cell strains and negatively correlated with prognosis, which can be very important biomarkers for HCC.
Growth and validation of a fast equipment for authenticity of murine meat in meat merchandise with a species-specific PCR assay
Adulteration of meat merchandise with murine meat poses an enormous risk to client well being and results in severe disruption in meals markets. Species authentication of murine meat remains to be technically difficult. We, due to this fact, developed a species-specific PCR equipment consisting of murine meat DNA extraction, PCR response and figuring out methods.
We designed novel common primers focusing on extremely conserved area on mitochondrial cytochrome b gene (cyt b) from 4 murines (lab rats, lab mice, wild rat and wild mice), in addition to particular primers for meat from 4 extensively consumed animal species, cattle, sheep, duck and donkey.
Concurrently, pasmid inserted particular cyt b fragment was cloned and used as the interior positve management within the equipment. The equipment parameters of specificity, sensitivity, stability and validity have been decided utilizing mimic counterfeiting meatball. The specificity of the DNA detection equipment was 100% in authentication of the 4 fraudulent meats of cattle, sheep, duck and donkey combined murine meat. The minimal detection restrict of the pattern DNA was 0.1 μg.
The equipment, which had freeze-thawed as much as 20 instances and saved for 1 12 months, additionally was highly effective in detecting an quantity of 0.1 mg in synthetic counterfeited cattle, sheep, duck and donkey meat merchandise. The murine-species DNA detection equipment proposed on this research has proved to be a easy, correct and efficient assay, and will be utilized to the identification of murine meat traces in frequent edible meat, to make sure the realisable implementation of meat product market supervision.
General Blocking Buffer-BB1 |
KF17337 |
Neuromics |
500 ml |
EUR 277.2 |
Neptune Blocking Buffer-BB2 |
KF17338 |
Neuromics |
500 ml |
EUR 292.8 |
Blocking Buffer, 10X, 250ML |
X109-250ML |
Arbor Assays |
250ML |
EUR 430.8 |
Blocking Buffer, 10X, 25ML |
X109-25ML |
Arbor Assays |
25ML |
EUR 146.4 |
EZBlock? (TBS) Blocking Buffer |
2117-1000 |
Biovision |
each |
EUR 254.4 |
EZBlock? (TBS) Blocking Buffer |
2117-200 |
Biovision |
each |
EUR 150 |
EZBlock? (PBS) Blocking Buffer |
2128-1000 |
Biovision |
each |
EUR 274.8 |
EZBlock? (PBS) Blocking Buffer |
2128-200 |
Biovision |
each |
EUR 164.4 |
Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed |
ELISA-1 |
Alpha Diagnostics |
1 |
EUR 242.4 |
TrueBlack WB Blocking Buffer Kit |
23013 |
Biotium |
1kit |
EUR 416.4 |
Description: Minimum order quantity: 1 unit of 1kit |
TrueBlack WB Blocking Buffer Kit |
23013-T |
Biotium |
1kit |
EUR 150 |
Description: Minimum order quantity: 1 unit of 1kit |
Blocking Buffer and Coating Stabilizer |
abx090811-1000ml |
Abbexa |
1000 ml |
EUR 627.6 |
|
3 Blocking Buffer Sample Pack |
KF17340 |
Neuromics |
3 x 100 ml |
EUR 246 |
TBS blocking buffer with BSA |
SD8113 |
Bio Basic |
1pk, 1L |
EUR 100.72 |
|
PBS Blocking buffer with BSA |
SB0626 |
Bio Basic |
1pk, 1L |
EUR 100.72 |
|
West-Ezier Super Blocking Buffer |
W3700-010 |
GenDepot |
100ml |
EUR 116.4 |
West-Ezier Super Blocking Buffer |
W3700-050 |
GenDepot |
500ml |
EUR 198 |
West-Ezier Super Blocking Buffer |
W3700-100 |
GenDepot |
1L |
EUR 295.2 |
EZBlock? T20 (TBS) Blocking Buffer |
2140-1000 |
Biovision |
each |
EUR 261.6 |
EZBlock? T20 (TBS) Blocking Buffer |
2140-200 |
Biovision |
each |
EUR 144 |
EZBlock? T20 (PBS) Blocking Buffer |
2143-1000 |
Biovision |
each |
EUR 261.6 |
EZBlock? T20 (PBS) Blocking Buffer |
2143-200 |
Biovision |
each |
EUR 144 |
ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml |
9068 |
Chondrex |
100 ml |
EUR 155.7 |
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer |
ELISA Plate Blocking Buffer concentrate (10X) BSA-based |
80060 |
Alpha Diagnostics |
50 ml |
EUR 242.4 |
Casein-based PBS Blocking Buffer Pack |
AR0182 |
BosterBio |
500mL/Pack |
EUR 84 |
Casein-based TBS Blocking Buffer Pack |
AR0183 |
BosterBio |
500mL/Pack |
EUR 84 |
Blocking Buffer for ICC and IHC |
SF40011 |
Neuromics |
10 ml |
EUR 153.6 |
EZBlock? Blocking Buffer and Signal Enhancer |
M4104-100 |
Biovision |
each |
EUR 183.6 |
EZBlock? Blocking Buffer and Signal Enhancer |
M4104-500 |
Biovision |
each |
EUR 430.8 |
Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers |
5730 |
Scientific Laboratory Supplies |
EACH |
EUR 864.12 |
Blocking Buffer for Lateral Flow (PBS based) |
abx090808-500ml |
Abbexa |
500 ml |
EUR 460.8 |
|
PBS Blocking buffer with non fat milk |
SB0625 |
Bio Basic |
1pk, 1L |
EUR 80.88 |
|
Blocking Buffer 1 (Various Epigenetics Assay Kits) |
79556 |
BPS Bioscience |
50 ml |
EUR 100 |
Description: This Blocking Buffer 1 is optimized for minimizing background in various BPS Bioscience assay kits. The BPS Bioscience Blocking Buffer improves S/B ratio of HMTs NSD2 and SetDB2 ELISAs with respect to competitor blocking buffer by both decreasing background (0% Activity control, no enzyme) and increasing assay signal (100% Activity control, enzyme without inhibitor). |
Blocking Buffer 2 (BPS Immunotherapy Assay Kits) |
79728 |
BPS Bioscience |
50 ml |
EUR 110 |
Blocking Buffer 3 (Multiple PARP Assay Kits) |
79743 |
BPS Bioscience |
25 ml x 2 |
EUR 105 |
Description: This Blocking Buffer 3 is optimized for minimizing background in multiple PARP BPS Bioscience Assay Kits. _x000D__x000D_ |
Blocking Buffer 4 (Multiple Epigenetics Assay Kits) |
52100 |
BPS Bioscience |
50 ml |
EUR 95 |
Description: This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays. |
West-Ezier Super Blocking Buffer for Phospho Antibody |
W3701-010 |
GenDepot |
100ml |
EUR 157.2 |
West-Ezier Super Blocking Buffer for Phospho Antibody |
W3701-050 |
GenDepot |
500ml |
EUR 225.6 |
West-Ezier Super Blocking Buffer for Phospho Antibody |
W3701-100 |
GenDepot |
1L |
EUR 336 |
High Performance ELISA Buffer |
85R-105 |
Fitzgerald |
55 ml |
EUR 379.2 |
Description: High Performance ELISA Buffer for high sensitivity ELISA assays |
Sandwich Elisa Wash Buffer |
RKB0001 |
Abclonal |
20ml |
EUR 142.8 |
West-Ez Blocking Buffer, 1 % Casein for Western Blot |
W3720-050 |
GenDepot |
500ml |
EUR 144 |
West-Ez Blocking Buffer, 1 % Casein for Western Blot |
W3720-100 |
GenDepot |
2x500ml |
EUR 176.4 |
Tricine buffer |
20-abx082551 |
Abbexa |
|
|
|
Wash Buffer |
abx098952-20ml |
Abbexa |
20 ml |
EUR 109.2 |
|
Coating Buffer |
abx098970-1vial |
Abbexa |
1 vial |
EUR 184.8 |
|
Lysis Buffer |
abx098984-LysisBuffer120ml |
Abbexa |
Lysis Buffer 1 (20 ml) |
EUR 184.8 |
|
Lysis Buffer |
abx098984-LysisBuffer3100ml |
Abbexa |
Lysis Buffer 3 (100ml) |
EUR 276 |
|
Lysis Buffer |
abx098984-LysisBuffer420ml |
Abbexa |
Lysis Buffer 4 (20 ml) |
EUR 184.8 |
|
Binding Buffer |
abx290019-50ml |
Abbexa |
50 ml |
EUR 276 |
|
Wash Buffer |
abx293002-30ml |
Abbexa |
30 ml |
EUR 126 |
|
Binding Buffer |
BB10X-50ML |
ImmunoStep |
50 ML |
EUR 136.44 |
Wash Buffer |
KF17356 |
Neuromics |
500 ml |
EUR 214.8 |
10xTaq Buffer |
PCRB60 |
Bio Basic |
4x1.5ml, 6ml |
EUR 70.44 |
|
PBS Buffer |
RM00012 |
Abclonal |
2L |
EUR 122.4 |
Dilution Buffer |
1066-100 |
Biovision |
each |
EUR 183.6 |
Dilution Buffer |
1066-400 |
Biovision |
each |
EUR 314.4 |
Wash Buffer |
1210-200 |
Biovision |
each |
EUR 235.2 |
RIPA Buffer |
2114-100 |
Biovision |
each |
EUR 189.6 |
RIPA Buffer |
2114-500 |
Biovision |
each |
EUR 555.6 |
CSB; Cytokine Stabilization Buffer ELISA |
CT373 |
U-CyTech |
5 ml |
EUR 114 |
West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST |
W3710-010 |
GenDepot |
100ml |
EUR 111.6 |
West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST |
W3710-050 |
GenDepot |
500ml |
EUR 165.6 |
West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST |
W3710-100 |
GenDepot |
1L |
EUR 218.4 |
West-Ezier Rapid Blocking Buffer, Ready-to-use, 5 Min |
W3871-050 |
GenDepot |
500ml |
EUR 230.4 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
T8052-050 |
GenDepot |
500ml |
EUR 96 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
T8052-100 |
GenDepot |
2X500ml |
EUR 124.8 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
T8052-101 |
GenDepot |
1L |
EUR 114 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
T8052-200 |
GenDepot |
4X500ml |
EUR 153.6 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
T8052-201 |
GenDepot |
2X1L |
EUR 153.6 |
10X Tris-Glycine Native Buffer (Transfer buffer) |
T8052-401 |
GenDepot |
4X1L |
EUR 198 |
TT Buffer (Tris-Tricine buffer) Primix powder |
TD8133 |
Bio Basic |
1PK, 10L |
EUR 91.32 |
|
Claudin Domain Containing 1 Blocking Peptide |
33R-9186 |
Fitzgerald |
100 ug |
EUR 216 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CLDND1 antibody, catalog no. 70R-6340 |
West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST |
W3711-010 |
GenDepot |
100ml |
EUR 116.4 |
West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST |
W3711-050 |
GenDepot |
500ml |
EUR 174 |
West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST |
W3711-100 |
GenDepot |
1L |
EUR 230.4 |
Calcium calibration buffer |
59100 |
Biotium |
1KIT |
EUR 261.6 |
Description: Minimum order quantity: 1 unit of 1KIT |
TBE Buffer, 5X |
41006 |
Biotium |
4L |
EUR 133.2 |
Description: Minimum order quantity: 1 unit of 4L |
Multianalyte azo dye as an on-site assay equipment for colorimetric detection of Hg2+ions and electrochemical sensing of Zn2+ ions
A brand new tailored colorimetric chemosensor, (E)-1-(benzo[d]thiazol-2-yl)-3-(pyridin-3-yldiazenyl)naphthalen-2-ol (1), containing benzothiazole and pyridine moieties related by an azo (-N=N-) linkage has been designed and synthesized. The synthesized chemosensor displayed an eye catching shade change upon binding to acetate [AcO–;colorless to russet] and mercury (II) [Hg2+;colorless to greenish blue] ions in 9:1 (v/v) aqueous CH3CN (pH 7.Zero HEPES buffer).
The mechanism of interplay between the chemosensor and the Hg2+/AcO– ions has been confirmed by 1H NMR titration experiments. Furthermore, the colorimetric chemosensor 1 displayed potential in-field purposes as on-site assay equipment and detection of Hg2+ ions in actual water samples. Importantly chemosensor 1 gave selective electrochemical response in direction of Zn2+ ions, enabling easy azo-dye 1 as multichannel chemosensor for colorimetric detection of Hg2+ ions and electrochemical detection of Zn2+ ions.
Comparability of Industrial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based mostly Immunoassay for Detecting a Urine-Based mostly Bladder-Most cancers-Related Diagnostic Signature.
The power to precisely measure a number of proteins concurrently in a single assay has the potential to markedly enhance the effectivity of medical checks composed of a number of biomarkers. We investigated the diagnostic accuracy of the 2 multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 topics (40 with bladder most cancers).
Banked urine samples collected from Kyoto and Nara Universities have been in comparison with histologically decided bladder most cancers. The concentrations of the 10 proteins (A1AT; apolipoprotein E-APOE; angiogenin-ANG; carbonic anhydrase 9-CA9; interleukin 8-IL-8; matrix metalloproteinase 9-MMP-9; matrix metalloproteinase 10-MMP10; plasminogen activator inhibitor 1-PAI-1; syndecan-SDC1; and vascular endothelial development factor-VEGF) have been monitored utilizing two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) in keeping with the producer’s technical specs.
The vary for detecting every biomarker was improved within the multiplex assays, regardless that the decrease restrict of quantification (LLOQ) was sometimes decrease within the business ELISA kits. The world underneath the receiver working traits (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based immunoassay (MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA). The sensitivities and specificities for MBA have been 0.93 and 0.95, respectively, and for MEA have been 0.85 and 0.80, respectively.
Accuracy, constructive predictive values (PPV), and unfavorable predictive values (NPV) for MBA have been 0.94, 0.95, and 0.93, respectively, and for MEA have been 0.83, 0.81, and 0.84, respectively. Based mostly on these encouraging preliminary information, we imagine {that a} multiplex protein array is a viable platform that may be utilized as an environment friendly and extremely correct device to quantitate a number of proteins inside biologic specimens.
Caspase 13 Assay Kit, green |
KF17208 |
Neuromics |
25 Tests |
EUR 364.8 |
Caspase 3 & 7 Assay Kit, green |
KF17203 |
Neuromics |
25 Tests |
EUR 364.8 |
OPTIONAL GREEN FILTER (572NM) |
GDBL-GREEN |
CORNING |
1/pk |
EUR 1262.4 |
Description: Lab Equipment; Axygen Branded EQ |
Caspase 1 Assay Kit |
20-abx299019 |
Abbexa |
|
|
|
Caspase 1 Assay Kit |
abx299023-2550tests |
Abbexa |
25-50 tests |
EUR 830.4 |
|
Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence* |
13503 |
AAT Bioquest |
500 Tests |
EUR 367.2 |
|
Cell Meter™ Live Cell Caspase 1 Binding Assay Kit *Green Fluorescence* |
20108 |
AAT Bioquest |
25 Tests |
EUR 367.2 |
|
Morpheus HT-96 Green Screen |
M-MD1-47-GREEN |
MiTeGen |
96 x 1 ml ml |
EUR 280 |
|
Description: Morpheus HT-96 Green Screen |
Polycaspase Assay Kit, green |
KF17200 |
Neuromics |
25 Tests |
EUR 364.8 |
Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit |
30009-1 |
Biotium |
1KIT |
EUR 120 |
Description: Minimum order quantity: 1 unit of 1KIT |
Caspase-8 IETD-R110 Fluorometric HTS Assay Kit |
30012-1 |
Biotium |
1KIT |
EUR 120 |
Description: Minimum order quantity: 1 unit of 1KIT |
Caspase 1 Fluorometric Assay Kit |
55R-1272 |
Fitzgerald |
25 assays |
EUR 332.4 |
Description: Assay Kit for detection of Capase 1 activity in the research laboratory |
Caspase 1 Colorimetric Assay Kit |
55R-1273 |
Fitzgerald |
25 assays |
EUR 332.4 |
Description: Assay Kit for detection of Capase 1 activity in the research laboratory |
Caspase-1 Activity Assay Kit |
AR4003-100Assays |
BosterBio |
100 Assays |
EUR 616.8 |
Caspase-1 Activity Assay Kit |
AR4003-25Assays |
BosterBio |
25 Assays |
EUR 283.2 |
Caspase-1 Activity Assay Kit |
AR4003-50Assays |
BosterBio |
50 Assays |
EUR 408 |
660 Caspase-1 Assay Kit |
KF17362 |
Neuromics |
25-50 Tests |
EUR 418.8 |
Caspase-1 Fluorometric Assay Kit |
K2010-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-1 Fluorometric Assay Kit |
K2010-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-1 Fluorometric Assay Kit |
K2010-400 |
ApexBio |
400 assays |
EUR 1303.2 |
Caspase-1 Colorimetric Assay Kit |
K2011-100 |
ApexBio |
100 assays |
EUR 568.8 |
Caspase-1 Colorimetric Assay Kit |
K2011-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-1 Colorimetric Assay Kit |
K2011-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-1 Colorimetric Assay Kit |
K2011-400 |
ApexBio |
400 assays |
EUR 1303.2 |
Caspase-1 Fluorometric Assay Kit |
K110-100 |
Biovision |
each |
EUR 574.8 |
Caspase-1 Fluorometric Assay Kit |
K110-200 |
Biovision |
each |
EUR 744 |
Caspase-1 Fluorometric Assay Kit |
K110-25 |
Biovision |
each |
EUR 248.4 |
Caspase-1 Fluorometric Assay Kit |
K110-400 |
Biovision |
each |
EUR 1149.6 |
Caspase-1 Colorimetric Assay Kit |
K111-100 |
Biovision |
each |
EUR 574.8 |
Caspase-1 Colorimetric Assay Kit |
K111-200 |
Biovision |
each |
EUR 744 |
Caspase-1 Colorimetric Assay Kit |
K111-25 |
Biovision |
each |
EUR 248.4 |
Caspase-1 Colorimetric Assay Kit |
K111-400 |
Biovision |
each |
EUR 1149.6 |
Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence* |
22798 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence* |
22799 |
AAT Bioquest |
200 Tests |
EUR 367.2 |
|
Malachite Green Phosphate Assay Kit |
abx298878-100Assays |
Abbexa |
100 Assays |
EUR 453.6 |
|
Malachite Green Phosphate Assay Kit |
POMG-25H |
BioAssay Systems |
2500 |
EUR 330 |
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C. |
Malachite Green Phosphate Assay Kit |
Z5030012 |
Biochain |
2,500 assays |
EUR 410.4 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Malachite Green Phosphate Assay kit |
65-POMG-25H |
Gentaur Genprice |
2500 tests |
EUR 333 |
HDAC Fluorogenic Assay Kit (Green) |
50034 |
BPS Bioscience |
96 rxns. |
EUR 450 |
Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening_x000D_and profiling applications. The kit comes in a convenient 96-well format, with all the_x000D_reagents necessary for 100 fluorescent HDAC activity measurements. In_x000D_addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor,_x000D_Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC_x000D_Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_steps on a microtiter plate are needed to analyze the HDAC activity level. First,_x000D_the HDAC fluorometric substrate, containing an acetylated lysine side chain, is_x000D_incubated with purified HDAC enzyme. The deacetylation sensitizes the_x000D_substrate so subsequent treatment with the Lysine Developer produces a_x000D_fluorophore that can then be measured using a fluorescence reader at 485 nm_x000D_(excitation)/528 nm (emission)._x000D__x000D_Our other HDAC kit (#50033) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However, the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate |
Cell Meter™ Live Cell Caspase 2 Binding Assay Kit *Green Fluorescence* |
20111 |
AAT Bioquest |
25 Tests |
EUR 367.2 |
|
Cell Meter™ Live Cell Caspase 6 Binding Assay Kit *Green Fluorescence* |
20113 |
AAT Bioquest |
25 Tests |
EUR 367.2 |
|
Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence* |
20115 |
AAT Bioquest |
25 Tests |
EUR 367.2 |
|
Cell Meter™ Live Cell Caspase 9 Binding Assay Kit *Green Fluorescence* |
20117 |
AAT Bioquest |
25 Tests |
EUR 367.2 |
|
Cell Meter™ Live Cell Caspase 10 Binding Assay Kit *Green Fluorescence* |
20119 |
AAT Bioquest |
25 Tests |
EUR 367.2 |
|
Cell Meter™ Live Cell Caspase 13 Binding Assay Kit *Green Fluorescence* |
20125 |
AAT Bioquest |
25 Tests |
EUR 367.2 |
|
Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence* |
22796 |
AAT Bioquest |
200 Tests |
EUR 262.8 |
|
Caspase 12 Assay Kit |
55R-1290 |
Fitzgerald |
25 assays |
EUR 393.6 |
Description: Assay Kit for detection of Capase 12 activity in the research laboratory |
Caspase 3 Assay Kit |
20-abx294003 |
Abbexa |
-
EUR 1506.00
-
EUR 548.40
-
EUR 924.00
|
- 100 tests
- 20 tests
- 50 tests
|
|
Poly-Caspase Assay Kit |
20-abx299016 |
Abbexa |
|
|
|
Caspase 6 Assay Kit |
20-abx299018 |
Abbexa |
|
|
|
Caspase 8 Assay Kit |
20-abx299020 |
Abbexa |
|
|
|
Caspase 9 Assay Kit |
20-abx299021 |
Abbexa |
|
|
|
Poly-Caspase Assay Kit |
abx299022-2550tests |
Abbexa |
25-50 tests |
EUR 830.4 |
|
Poly-Caspase Assay Kit |
20-abx299025 |
Abbexa |
|
|
|
Caspase 2 Assay Kit |
abx299026-25tests |
Abbexa |
25 tests |
EUR 698.4 |
|
Caspase 10 Assay Kit |
abx299027-100tests |
Abbexa |
100 tests |
EUR 1562.4 |
|
Caspase 9 Assay Kit |
20-abx299035 |
Abbexa |
|
|
|
Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays) |
30011-1 |
Biotium |
1KIT |
EUR 244.8 |
Description: Minimum order quantity: 1 unit of 1KIT |
Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence* |
20100 |
AAT Bioquest |
25 Tests |
EUR 367.2 |
|
EZCell? Phagocytosis Assay Kit (Green Zymosan) |
K397-100 |
Biovision |
each |
EUR 516 |
EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit |
P-3001 |
EpiGentek |
-
EUR 1050.06
-
EUR 424.60
-
EUR 712.80
|
- 96 Assays
- 48 Assays
- 96 Assays
|
EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K4) |
P-3002 |
EpiGentek |
-
EUR 1067.46
-
EUR 437.80
-
EUR 724.90
|
- 96 Assays
- 48 Assays
- 96 Assays
|
EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K9) |
P-3003 |
EpiGentek |
-
EUR 1067.46
-
EUR 437.80
-
EUR 724.90
|
- 96 Assays
- 48 Assays
- 96 Assays
|
Caspase 3 Fluorometric Assay Kit |
55R-1269 |
Fitzgerald |
25 assays |
EUR 332.4 |
Description: Assay Kit for detection of Capase 3 activity in the research laboratory |
Caspase 3 Colorimetric Assay Kit |
55R-1270 |
Fitzgerald |
25 assays |
EUR 332.4 |
Description: Assay Kit for detection of Capase 3 activity in the research laboratory |
Caspase 8 Fluorometric Assay Kit |
55R-1274 |
Fitzgerald |
25 assays |
EUR 332.4 |
Description: Assay Kit for detection of Capase 8 activity in the research laboratory |
Caspase 8 Colorimetric Assay Kit |
55R-1275 |
Fitzgerald |
25 assays |
EUR 332.4 |
Description: Assay Kit for detection of Capase 8 activity in the research laboratory |
Caspase 6 Fluorometric Assay Kit |
55R-1276 |
Fitzgerald |
25 assays |
EUR 344.4 |
Description: Assay Kit for detection of Capase 6 activity in the research laboratory |
Caspase 6 Colorimetric Assay Kit |
55R-1277 |
Fitzgerald |
25 assays |
EUR 340.8 |
Description: Assay Kit for detection of Capase 6 activity in the research laboratory |
Caspase 2 Fluorometric Assay Kit |
55R-1278 |
Fitzgerald |
25 assays |
EUR 340.8 |
Description: Assay Kit for detection of Capase 2 activity in the research laboratory |
Caspase 2 Colorimetric Assay Kit |
55R-1279 |
Fitzgerald |
25 assays |
EUR 363.6 |
Description: Assay Kit for detection of Capase 2 activity in the research laboratory |
Caspase 9 Fluorometric Assay Kit |
55R-1280 |
Fitzgerald |
25 assays |
EUR 332.4 |
Description: Assay Kit for detection of Capase 9 activity in the research laboratory |
Caspase 9 Colorimetric Assay Kit |
55R-1281 |
Fitzgerald |
25 assays |
EUR 355.2 |
Description: Assay Kit for detection of Capase 9 activity in the research laboratory |
Caspase 5 Fluorometric Assay Kit |
55R-1282 |
Fitzgerald |
25 assays |
EUR 340.8 |
Description: Assay Kit for detection of Capase 5 activity in the research laboratory |
Caspase 5 Colorimetric Assay Kit |
55R-1283 |
Fitzgerald |
25 assays |
EUR 363.6 |
Description: Assay Kit for detection of Capase 5 activity in the research laboratory |
Caspase 10 Fluorometric Assay Kit |
55R-1284 |
Fitzgerald |
25 assays |
EUR 340.8 |
Description: Assay Kit for detection of Capase 10 activity in the research laboratory |
Caspase 10 Colorimetric Assay Kit |
55R-1285 |
Fitzgerald |
25 assays |
EUR 363.6 |
Description: Assay Kit for detection of Capase 10 activity in the research laboratory |
Caspase 4 Fluorometric Assay Kit |
55R-1286 |
Fitzgerald |
25 assays |
EUR 363.6 |
Description: Assay Kit for detection of Capase 4 activity in the research laboratory |
Caspase 4 Colorimetric Assay Kit |
55R-1287 |
Fitzgerald |
25 assays |
EUR 374.4 |
Description: Assay Kit for detection of Capase 4 activity in the research laboratory |
Caspase 3/7 Assay Kit |
20-abx299017 |
Abbexa |
|
|
|
Caspase 3/7 Assay Kit |
abx299024-2550tests |
Abbexa |
25-50 tests |
EUR 830.4 |
|
Caspase 3/7 Assay Kit |
20-abx299028 |
Abbexa |
|
|
|
Caspase-2 Activity Assay Kit |
AR4004-100Assays |
BosterBio |
100 Assays |
EUR 616.8 |
Caspase-2 Activity Assay Kit |
AR4004-25Assays |
BosterBio |
25 Assays |
EUR 283.2 |
Caspase-2 Activity Assay Kit |
AR4004-50Assays |
BosterBio |
50 Assays |
EUR 408 |
QuantiFluo Caspase-3 Assay Kit |
DCS3-100 |
BioAssay Systems |
100 |
EUR 330 |
Description: Quantitative determination of the apoptosis target caspase-3 activity by fluorimetry (400/490nm) method. Procedure: 60 min. Kit size: 100 tests. Shelf life: 12 months. |
Caspase-5 Fluorometric Assay Kit |
K2195-100 |
ApexBio |
100 assays |
EUR 602.4 |
Caspase-5 Fluorometric Assay Kit |
K2195-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-5 Fluorometric Assay Kit |
K2195-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-5 Fluorometric Assay Kit |
K2195-400 |
ApexBio |
400 assays |
EUR 1336.8 |
Caspase-5 Colorimetric Assay Kit |
K2196-100 |
ApexBio |
100 assays |
EUR 634.8 |
Caspase-5 Colorimetric Assay Kit |
K2196-200 |
ApexBio |
200 assays |
EUR 870 |
Caspase-5 Colorimetric Assay Kit |
K2196-25 |
ApexBio |
25 assays |
EUR 301.2 |
Caspase-5 Colorimetric Assay Kit |
K2196-400 |
ApexBio |
400 assays |
EUR 1387.2 |
Caspase-10 Colorimetric Assay Kit |
K2197-100 |
ApexBio |
100 assays |
EUR 634.8 |
Caspase-10 Colorimetric Assay Kit |
K2197-200 |
ApexBio |
200 assays |
EUR 885.6 |
Caspase-10 Colorimetric Assay Kit |
K2197-25 |
ApexBio |
25 assays |
EUR 301.2 |
Caspase-10 Colorimetric Assay Kit |
K2197-400 |
ApexBio |
400 assays |
EUR 1336.8 |
Caspase-4 Fluorometric Assay Kit |
K2198-100 |
ApexBio |
100 assays |
EUR 634.8 |
Caspase-4 Fluorometric Assay Kit |
K2198-200 |
ApexBio |
200 assays |
EUR 885.6 |
Caspase-4 Fluorometric Assay Kit |
K2198-25 |
ApexBio |
25 assays |
EUR 301.2 |
Caspase-4 Fluorometric Assay Kit |
K2198-400 |
ApexBio |
400 assays |
EUR 1336.8 |
Caspase-4 Colorimetric Assay Kit |
K2199-100 |
ApexBio |
100 assays |
EUR 668.4 |
Caspase-4 Colorimetric Assay Kit |
K2199-200 |
ApexBio |
200 assays |
EUR 885.6 |
Caspase-4 Colorimetric Assay Kit |
K2199-25 |
ApexBio |
25 assays |
EUR 319.2 |
Caspase-4 Colorimetric Assay Kit |
K2199-400 |
ApexBio |
400 assays |
EUR 1336.8 |
Caspase 9 Assay Kit, red |
KF17302 |
Neuromics |
25 Tests |
EUR 364.8 |
Caspase-3 Fluorometric Assay Kit |
K2007-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-3 Fluorometric Assay Kit |
K2007-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-3 Fluorometric Assay Kit |
K2007-400 |
ApexBio |
400 assays |
EUR 1303.2 |
Caspase-3 Colorimetric Assay Kit |
K2008-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-3 Colorimetric Assay Kit |
K2008-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-3 Colorimetric Assay Kit |
K2008-400 |
ApexBio |
400 assays |
EUR 1303.2 |
Caspase-8 Fluorometric Assay Kit |
K2012-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-8 Fluorometric Assay Kit |
K2012-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-8 Fluorometric Assay Kit |
K2012-400 |
ApexBio |
400 assays |
EUR 1303.2 |
Caspase-8 Colorimetric Assay Kit |
K2013-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-8 Colorimetric Assay Kit |
K2013-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-8 Colorimetric Assay Kit |
K2013-400 |
ApexBio |
400 assays |
EUR 1303.2 |
Caspase-6 Fluorometric Assay Kit |
K2014-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-6 Fluorometric Assay Kit |
K2014-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-6 Fluorometric Assay Kit |
K2014-400 |
ApexBio |
400 assays |
EUR 1303.2 |
Caspase-6 Colorimetric Assay Kit |
K2015-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-6 Colorimetric Assay Kit |
K2015-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-6 Colorimetric Assay Kit |
K2015-400 |
ApexBio |
400 assays |
EUR 1321.2 |
Caspase-2 Fluorometric Assay Kit |
K2016-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-2 Fluorometric Assay Kit |
K2016-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-2 Fluorometric Assay Kit |
K2016-400 |
ApexBio |
400 assays |
EUR 1336.8 |
Caspase-2 Colorimetric Assay Kit |
K2017-200 |
ApexBio |
200 assays |
EUR 870 |
Caspase-2 Colorimetric Assay Kit |
K2017-25 |
ApexBio |
25 assays |
EUR 301.2 |
Caspase-2 Colorimetric Assay Kit |
K2017-400 |
ApexBio |
400 assays |
EUR 1387.2 |
Caspase-9 Fluorometric Assay Kit |
K2018-200 |
ApexBio |
200 assays |
EUR 802.8 |
Caspase-9 Fluorometric Assay Kit |
K2018-25 |
ApexBio |
25 assays |
EUR 285.6 |
Caspase-9 Fluorometric Assay Kit |
K2018-400 |
ApexBio |
400 assays |
EUR 1336.8 |
Caspase-9 Colorimetric Assay Kit |
K2019-200 |
ApexBio |
200 assays |
EUR 835.2 |
Caspase-9 Colorimetric Assay Kit |
K2019-25 |
ApexBio |
25 assays |
EUR 301.2 |
Caspase-9 Colorimetric Assay Kit |
K2019-400 |
ApexBio |
400 assays |
EUR 1387.2 |
Caspase-12 Fluorometric Assay Kit |
K2150-100 |
ApexBio |
100 assays |
EUR 634.8 |
Caspase-12 Fluorometric Assay Kit |
K2150-25 |
ApexBio |
25 assays |
EUR 301.2 |
Caspase-3 Fluorometric Assay Kit |
K105-100 |
Biovision |
each |
EUR 574.8 |
Caspase-3 Fluorometric Assay Kit |
K105-200 |
Biovision |
each |
EUR 744 |
Caspase-3 Fluorometric Assay Kit |
K105-25 |
Biovision |
each |
EUR 248.4 |
Caspase-3 Fluorometric Assay Kit |
K105-400 |
Biovision |
each |
EUR 1174.8 |
Caspase-3 Colorimetric Assay Kit |
K106-100 |
Biovision |
each |
EUR 574.8 |
Caspase-3 Colorimetric Assay Kit |
K106-200 |
Biovision |
each |
EUR 757.2 |
Caspase-3 Colorimetric Assay Kit |
K106-25 |
Biovision |
each |
EUR 248.4 |
Caspase-3 Colorimetric Assay Kit |
K106-400 |
Biovision |
each |
EUR 1149.6 |
Caspase-8 Fluorometric Assay Kit |
K112-100 |
Biovision |
each |
EUR 574.8 |