Distinct contributions of DNA methylation and histone acetylation to the genomic occupancy of transcription components

Epigenetic modifications on chromatin play very important roles in regulating gene expression. Although chromatin states are typically dominated by multilayered development, how explicit individual pathways contribute to gene expression stays poorly understood. As an example, DNA methylation is known to handle transcription subject binding however along with recruit methyl-CpG binding proteins that impact chromatin development by the train of histone deacetylase complexes (HDACs).

Every of these mechanisms can most likely impact gene expression, nevertheless the importance of each, and whether or not or not these actions are built-in to appreciate acceptable gene regulation, stays largely unknown.

To take care of this very important question, we measured gene expression, chromatin accessibility, and transcription subject occupancy in wild-type or DNA methylation-deficient mouse embryonic stem cells following HDAC inhibition.

We observe widespread will enhance in chromatin accessibility at retrotransposons when HDACs are inhibited, and that’s magnified when cells moreover lack DNA methylation. A subset of these elements has elevated binding of the YY1 and GABPA transcription elements and elevated expression.

The pronounced additive affect of HDAC inhibition in DNA methylation-deficient cells demonstrates that DNA methylation and histone deacetylation act largely independently to suppress transcription subject binding and gene expression.

Identification of the DNA Replication Regulator MCM Superior Expression and Prognostic Significance in Hepatic Carcinoma 

Background: The microliposome maintenance (MCM) superior, MCM2-7, is revealed to be involved in a lot of cell processes and performs a key place inside the development and growth of human cancers. Nonetheless, the MCM superior stays poorly elaborated in hepatic carcinoma (HCC).

Methods: Throughout the analysis, we found the mRNA and protein diploma by bioinformatics. We moreover explored the prognostic value, genetic alteration, interaction neighborhood, and sensible enrichment of MCM2-7. The MCM expression and correlation amongst these MCMs in HCC cell strains have been acknowledged by western blot.

Outcomes: MCM2-7 was significantly elevated in HCC tissues as compared with common liver tissues. The extreme diploma of MCM2-7 had a constructive correlation with poor prognosis. Nonetheless, MCM2-7 alterations weren’t correlated with poor OS. MCMs have been every elevated in HCC cell strains as compared with the traditional hepatocyte cell line. Furthermore, the constructive correlation was found amongst MCMs in HCC cell strains.

Conclusions: The MCM superior was elevated in HCC tissues and cell strains and negatively correlated with prognosis, which can be very important biomarkers for HCC.

Growth and validation of a fast equipment for authenticity of murine meat in meat merchandise with a species-specific PCR assay 

Adulteration of meat merchandise with murine meat poses an enormous risk to client well being and results in severe disruption in meals markets. Species authentication of murine meat remains to be technically difficult. We, due to this fact, developed a species-specific PCR equipment consisting of murine meat DNA extraction, PCR response and figuring out methods.

We designed novel common primers focusing on extremely conserved area on mitochondrial cytochrome b gene (cyt b) from 4 murines (lab rats, lab mice, wild rat and wild mice), in addition to particular primers for meat from 4 extensively consumed animal species, cattle, sheep, duck and donkey.

Concurrently, pasmid inserted particular cyt b fragment was cloned and used as the interior positve management within the equipment. The equipment parameters of specificity, sensitivity, stability and validity have been decided utilizing mimic counterfeiting meatball. The specificity of the DNA detection equipment was 100% in authentication of the 4 fraudulent meats of cattle, sheep, duck and donkey combined murine meat. The minimal detection restrict of the pattern DNA was 0.1 μg.

The equipment, which had freeze-thawed as much as 20 instances and saved for 1 12 months, additionally was highly effective in detecting an quantity of 0.1 mg in synthetic counterfeited cattle, sheep, duck and donkey meat merchandise. The murine-species DNA detection equipment proposed on this research has proved to be a easy, correct and efficient assay, and will be utilized to the identification of murine meat traces in frequent edible meat, to make sure the realisable implementation of meat product market supervision.

 

General Blocking Buffer-BB1
KF17337	Neuromics	500 ml	EUR 277.2

Neptune Blocking Buffer-BB2
KF17338	Neuromics	500 ml	EUR 292.8

Blocking Buffer, 10X, 250ML
X109-250ML	Arbor Assays	250ML	EUR 430.8

Blocking Buffer, 10X, 25ML
X109-25ML	Arbor Assays	25ML	EUR 146.4

EZBlock? (TBS) Blocking Buffer
2117-1000	Biovision	each	EUR 254.4

EZBlock? (TBS) Blocking Buffer
2117-200	Biovision	each	EUR 150

EZBlock? (PBS) Blocking Buffer
2128-1000	Biovision	each	EUR 274.8

EZBlock? (PBS) Blocking Buffer
2128-200	Biovision	each	EUR 164.4

Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed
ELISA-1	Alpha Diagnostics	1	EUR 242.4

TrueBlack WB Blocking Buffer Kit
23013	Biotium	1kit	EUR 416.4
Description: Minimum order quantity: 1 unit of 1kit

TrueBlack WB Blocking Buffer Kit
23013-T	Biotium	1kit	EUR 150
Description: Minimum order quantity: 1 unit of 1kit

Blocking Buffer and Coating Stabilizer
abx090811-1000ml	Abbexa	1000 ml	EUR 627.6

3 Blocking Buffer Sample Pack
KF17340	Neuromics	3 x 100 ml	EUR 246

10 x Universal Blocking Buffer
GR103005	Genorise Scientific	200 mL	EUR 154.8

TBS blocking buffer with BSA
SD8113	Bio Basic	1pk, 1L	EUR 100.72

PBS Blocking buffer with BSA
SB0626	Bio Basic	1pk, 1L	EUR 100.72

West-Ezier Super Blocking Buffer
W3700-010	GenDepot	100ml	EUR 116.4

West-Ezier Super Blocking Buffer
W3700-050	GenDepot	500ml	EUR 198

West-Ezier Super Blocking Buffer
W3700-100	GenDepot	1L	EUR 295.2

EZBlock? T20 (TBS) Blocking Buffer
2140-1000	Biovision	each	EUR 261.6

EZBlock? T20 (TBS) Blocking Buffer
2140-200	Biovision	each	EUR 144

EZBlock? T20 (PBS) Blocking Buffer
2143-1000	Biovision	each	EUR 261.6

EZBlock? T20 (PBS) Blocking Buffer
2143-200	Biovision	each	EUR 144

ChonBlock Blocking/Sample Dilution ELISA Buffer, 100 ml
9068	Chondrex	100 ml	EUR 155.7
Description: ChonBlock Blocking/Sample Dilution ELISA Buffer

ELISA Plate Blocking Buffer concentrate (10X) BSA-based
80060	Alpha Diagnostics	50 ml	EUR 242.4

Casein-based PBS Blocking Buffer Pack
AR0182	BosterBio	500mL/Pack	EUR 84

Casein-based TBS Blocking Buffer Pack
AR0183	BosterBio	500mL/Pack	EUR 84

Blocking Buffer for ICC and IHC
SF40011	Neuromics	10 ml	EUR 153.6

Corning 3D Clear Blocking Buffer - 30mL
5736	Scientific Laboratory Supplies	EACH	EUR 108.3

Corning 3D Clear Blocking Buffer - 100mL
5737	Scientific Laboratory Supplies	EACH	EUR 294.12

EZBlock? Blocking Buffer and Signal Enhancer
M4104-100	Biovision	each	EUR 183.6

EZBlock? Blocking Buffer and Signal Enhancer
M4104-500	Biovision	each	EUR 430.8

Corning 3D Clear Starter Kit - Includes Reagent/Antibody Buffer/Blocking Buffer/Penetration Buffer/Washing Buffers
5730	Scientific Laboratory Supplies	EACH	EUR 864.12

Blocking Buffer for Lateral Flow (PBS based)
abx090808-500ml	Abbexa	500 ml	EUR 460.8

T-Pro Fast Blocking Buffer (in PBS)
JK92-W001	T-Pro Biotechnology	500ml/BT	EUR 224.4

T-Pro Fast Blocking Buffer (in TBS)
JK92-W002	T-Pro Biotechnology	500ml/BT	EUR 224.4

PBS Blocking buffer with non fat milk
SB0625	Bio Basic	1pk, 1L	EUR 80.88

Blocking Buffer 1 (Various Epigenetics Assay Kits)
79556	BPS Bioscience	50 ml	EUR 100
Description: This Blocking Buffer 1 is optimized for minimizing background in various BPS Bioscience assay kits. The BPS Bioscience Blocking Buffer improves S/B ratio of HMTs NSD2 and SetDB2 ELISAs with respect to competitor blocking buffer by both decreasing background (0% Activity control, no enzyme) and increasing assay signal (100% Activity control, enzyme without inhibitor).

Blocking Buffer 2 (BPS Immunotherapy Assay Kits)
79728	BPS Bioscience	50 ml	EUR 110

Blocking Buffer 3 (Multiple PARP Assay Kits)
79743	BPS Bioscience	25 ml x 2	EUR 105
Description: This Blocking Buffer 3 is optimized for minimizing background in multiple PARP BPS Bioscience Assay Kits. _x000D__x000D_

Blocking Buffer 4 (Multiple Epigenetics Assay Kits)
52100	BPS Bioscience	50 ml	EUR 95
Description: This Blocking Buffer 4 is optimized for minimizing background in various chemiluminescent methyltransferase, demethylase, and TET assays.

ELISA Binding Buffer
EBB125	ScyTek Laboratories	125 ml	EUR 76.8

ELISA Binding Buffer
EBB500	ScyTek Laboratories	500 ml	EUR 104.4

ELISA Binding Buffer
EBB999	ScyTek Laboratories	1000 ml	EUR 129.6

T-Pro Protein Free Blocking Buffer (in PBS)
JK92-W003	T-Pro Biotechnology	500ml/BT	EUR 224.4

T-Pro Protein Free Blocking Buffer (in TBS)
JK92-W004	T-Pro Biotechnology	500ml/BT	EUR 224.4

West-Ezier Super Blocking Buffer for Phospho Antibody
W3701-010	GenDepot	100ml	EUR 157.2

West-Ezier Super Blocking Buffer for Phospho Antibody
W3701-050	GenDepot	500ml	EUR 225.6

West-Ezier Super Blocking Buffer for Phospho Antibody
W3701-100	GenDepot	1L	EUR 336

High Performance ELISA Buffer
85R-105	Fitzgerald	55 ml	EUR 379.2
Description: High Performance ELISA Buffer for high sensitivity ELISA assays

ELISA Assay Buffer, 20x
GR103001	Genorise Scientific	1 L	EUR 106.8

ELISA Assay Buffer, 80x
GR103002	Genorise Scientific	1 L	EUR 190.8

EIA Buffer for ELISA
GR103069	Genorise Scientific	100 mL	EUR 82.8

Sandwich Elisa Wash Buffer
RKB0001	Abclonal	20ml	EUR 142.8

West-Ez Blocking Buffer, 1 % Casein for Western Blot
W3720-050	GenDepot	500ml	EUR 144

West-Ez Blocking Buffer, 1 % Casein for Western Blot
W3720-100	GenDepot	2x500ml	EUR 176.4

Tricine buffer
20-abx082551	Abbexa	EUR 260.40 EUR 226.80	100 g 25 g

Wash Buffer
abx098952-20ml	Abbexa	20 ml	EUR 109.2

Coating Buffer
abx098970-1vial	Abbexa	1 vial	EUR 184.8

Lysis Buffer
abx098984-LysisBuffer120ml	Abbexa	Lysis Buffer 1 (20 ml)	EUR 184.8

Lysis Buffer
abx098984-LysisBuffer3100ml	Abbexa	Lysis Buffer 3 (100ml)	EUR 276

Lysis Buffer
abx098984-LysisBuffer420ml	Abbexa	Lysis Buffer 4 (20 ml)	EUR 184.8

Binding Buffer
abx290019-50ml	Abbexa	50 ml	EUR 276

Wash Buffer
abx293002-30ml	Abbexa	30 ml	EUR 126

Binding Buffer
BB10X-50ML	ImmunoStep	50 ML	EUR 136.44

ADA buffer
AD0003	Bio Basic	25g	EUR 77.75

Wash Buffer
KF17356	Neuromics	500 ml	EUR 214.8

RIPA Buffer
GR103019	Genorise Scientific	50 mL	EUR 70.8

Stripping Buffer
GR103020	Genorise Scientific	500 mL	EUR 106.8

Anode Buffer
GR103036	Genorise Scientific	1 L	EUR 106.8

Cathode Buffer
GR103037	Genorise Scientific	1 L	EUR 130.8

10xTaq Buffer
PCRB60	Bio Basic	4x1.5ml, 6ml	EUR 70.44

PBS Buffer
RM00012	Abclonal	2L	EUR 122.4

Phosphate Buffer
PB1	Scientific Laboratory Supplies	1L	EUR 94.62

Dilution Buffer
1066-100	Biovision	each	EUR 183.6

Dilution Buffer
1066-400	Biovision	each	EUR 314.4

Wash Buffer
1210-200	Biovision	each	EUR 235.2

RIPA Buffer
2114-100	Biovision	each	EUR 189.6

RIPA Buffer
2114-500	Biovision	each	EUR 555.6

CSB; Cytokine Stabilization Buffer ELISA
CT373	U-CyTech	5 ml	EUR 114

10 x ELISA Wash Buffer
GR103014	Genorise Scientific	1 L	EUR 117.6

20 x ELISA Wash Buffer
GR103014-20	Genorise Scientific	1 L	EUR 189.6

40 x ELISA Wash Buffer
GR103014-40	Genorise Scientific	1 L	EUR 238.8

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST
W3710-010	GenDepot	100ml	EUR 111.6

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST
W3710-050	GenDepot	500ml	EUR 165.6

West-Ez Blocking Buffer, 3 % IgG Free-BSA in TBST
W3710-100	GenDepot	1L	EUR 218.4

West-Ezier Rapid Blocking Buffer, Ready-to-use, 5 Min
W3871-050	GenDepot	500ml	EUR 230.4

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-050	GenDepot	500ml	EUR 96

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-100	GenDepot	2X500ml	EUR 124.8

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-101	GenDepot	1L	EUR 114

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-200	GenDepot	4X500ml	EUR 153.6

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-201	GenDepot	2X1L	EUR 153.6

10X Tris-Glycine Native Buffer (Transfer buffer)
T8052-401	GenDepot	4X1L	EUR 198

TT Buffer (Tris-Tricine buffer) Primix powder
TD8133	Bio Basic	1PK, 10L	EUR 91.32

Claudin Domain Containing 1 Blocking Peptide
33R-9186	Fitzgerald	100 ug	EUR 216
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of CLDND1 antibody, catalog no. 70R-6340

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST
W3711-010	GenDepot	100ml	EUR 116.4

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST
W3711-050	GenDepot	500ml	EUR 174

West-Ez Blocking Buffer, 3 % IgG & Fat Free-BSA in TBST
W3711-100	GenDepot	1L	EUR 230.4

Calcium calibration buffer
59100	Biotium	1KIT	EUR 261.6
Description: Minimum order quantity: 1 unit of 1KIT

TBE Buffer, 5X
41006	Biotium	4L	EUR 133.2
Description: Minimum order quantity: 1 unit of 4L

Multianalyte azo dye as an on-site assay equipment for colorimetric detection of Hg2+ions and electrochemical sensing of Zn2+ ions 

A brand new tailored colorimetric chemosensor, (E)-1-(benzo[d]thiazol-2-yl)-3-(pyridin-3-yldiazenyl)naphthalen-2-ol (1), containing benzothiazole and pyridine moieties related by an azo (-N=N-) linkage has been designed and synthesized. The synthesized chemosensor displayed an eye catching shade change upon binding to acetate [AcO^–;colorless to russet] and mercury (II) [Hg²⁺;colorless to greenish blue] ions in 9:1 (v/v) aqueous CH₃CN (pH 7.Zero HEPES buffer).

The mechanism of interplay between the chemosensor and the Hg²⁺/AcO^– ions has been confirmed by ¹H NMR titration experiments. Furthermore, the colorimetric chemosensor 1 displayed potential in-field purposes as on-site assay equipment and detection of Hg²⁺ ions in actual water samples. Importantly chemosensor 1 gave selective electrochemical response in direction of Zn²⁺ ions, enabling easy azo-dye 1 as multichannel chemosensor for colorimetric detection of Hg²⁺ ions and electrochemical detection of Zn²⁺ ions.

Comparability of Industrial ELISA Kits, a Prototype Multiplex Electrochemoluminescent Assay, and a Multiplex Bead-Based mostly Immunoassay for Detecting a Urine-Based mostly Bladder-Most cancers-Related Diagnostic Signature.

The power to precisely measure a number of proteins concurrently in a single assay has the potential to markedly enhance the effectivity of medical checks composed of a number of biomarkers. We investigated the diagnostic accuracy of the 2 multiplex protein array platforms for detecting a bladder-cancer-associated diagnostic signature in samples from a cohort of 80 topics (40 with bladder most cancers).

Banked urine samples collected from Kyoto and Nara Universities have been in comparison with histologically decided bladder most cancers. The concentrations of the 10 proteins (A1AT; apolipoprotein E-APOE; angiogenin-ANG; carbonic anhydrase 9-CA9; interleukin 8-IL-8; matrix metalloproteinase 9-MMP-9; matrix metalloproteinase 10-MMP10; plasminogen activator inhibitor 1-PAI-1; syndecan-SDC1; and vascular endothelial development factor-VEGF) have been monitored utilizing two prototype multiplex array platforms and an enzyme-linked immunosorbent assay (ELISA) in keeping with the producer’s technical specs.

The vary for detecting every biomarker was improved within the multiplex assays, regardless that the decrease restrict of quantification (LLOQ) was sometimes decrease within the business ELISA kits. The world underneath the receiver working traits (AUROC) of the prototype multiplex assays was reported to be 0.97 for the multiplex bead-based immunoassay (MBA) and 0.86 for the multiplex electrochemoluminescent assay (MEA). The sensitivities and specificities for MBA have been 0.93 and 0.95, respectively, and for MEA have been 0.85 and 0.80, respectively.

Accuracy, constructive predictive values (PPV), and unfavorable predictive values (NPV) for MBA have been 0.94, 0.95, and 0.93, respectively, and for MEA have been 0.83, 0.81, and 0.84, respectively. Based mostly on these encouraging preliminary information, we imagine {that a} multiplex protein array is a viable platform that may be utilized as an environment friendly and extremely correct device to quantitate a number of proteins inside biologic specimens.

Caspase 13 Assay Kit, green
KF17208	Neuromics	25 Tests	EUR 364.8

Caspase 3 & 7 Assay Kit, green
KF17203	Neuromics	25 Tests	EUR 364.8

OPTIONAL GREEN FILTER (572NM)
GDBL-GREEN	CORNING	1/pk	EUR 1262.4
Description: Lab Equipment; Axygen Branded EQ

Caspase 1 Assay Kit
20-abx299019	Abbexa	EUR 1638.00 EUR 754.80	100 tests 25 tests

Caspase 1 Assay Kit
abx299023-2550tests	Abbexa	25-50 tests	EUR 830.4

Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence*
13503	AAT Bioquest	500 Tests	EUR 367.2

Cell Meter™ Live Cell Caspase 1 Binding Assay Kit *Green Fluorescence*
20108	AAT Bioquest	25 Tests	EUR 367.2

Morpheus HT-96 Green Screen
M-MD1-47-GREEN	MiTeGen	96 x 1 ml ml	EUR 280
Description: Morpheus HT-96 Green Screen

Polycaspase Assay Kit, green
KF17200	Neuromics	25 Tests	EUR 364.8

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit
30009-1	Biotium	1KIT	EUR 120
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric HTS Assay Kit
30012-1	Biotium	1KIT	EUR 120
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 1 Fluorometric Assay Kit
55R-1272	Fitzgerald	25 assays	EUR 332.4
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit
55R-1273	Fitzgerald	25 assays	EUR 332.4
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase-1 Activity Assay Kit
AR4003-100Assays	BosterBio	100 Assays	EUR 616.8

Caspase-1 Activity Assay Kit
AR4003-25Assays	BosterBio	25 Assays	EUR 283.2

Caspase-1 Activity Assay Kit
AR4003-50Assays	BosterBio	50 Assays	EUR 408

660 Caspase-1 Assay Kit
KF17362	Neuromics	25-50 Tests	EUR 418.8

Caspase-1 Fluorometric Assay Kit
K2010-200	ApexBio	200 assays	EUR 835.2

Caspase-1 Fluorometric Assay Kit
K2010-25	ApexBio	25 assays	EUR 285.6

Caspase-1 Fluorometric Assay Kit
K2010-400	ApexBio	400 assays	EUR 1303.2

Caspase-1 Colorimetric Assay Kit
K2011-100	ApexBio	100 assays	EUR 568.8

Caspase-1 Colorimetric Assay Kit
K2011-200	ApexBio	200 assays	EUR 835.2

Caspase-1 Colorimetric Assay Kit
K2011-25	ApexBio	25 assays	EUR 285.6

Caspase-1 Colorimetric Assay Kit
K2011-400	ApexBio	400 assays	EUR 1303.2

Caspase-1 Fluorometric Assay Kit
K110-100	Biovision	each	EUR 574.8

Caspase-1 Fluorometric Assay Kit
K110-200	Biovision	each	EUR 744

Caspase-1 Fluorometric Assay Kit
K110-25	Biovision	each	EUR 248.4

Caspase-1 Fluorometric Assay Kit
K110-400	Biovision	each	EUR 1149.6

Caspase-1 Colorimetric Assay Kit
K111-100	Biovision	each	EUR 574.8

Caspase-1 Colorimetric Assay Kit
K111-200	Biovision	each	EUR 744

Caspase-1 Colorimetric Assay Kit
K111-25	Biovision	each	EUR 248.4

Caspase-1 Colorimetric Assay Kit
K111-400	Biovision	each	EUR 1149.6

Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*
22798	AAT Bioquest	200 Tests	EUR 262.8

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*
22799	AAT Bioquest	200 Tests	EUR 367.2

Malachite Green Phosphate Assay Kit
abx298878-100Assays	Abbexa	100 Assays	EUR 453.6

Malachite Green Phosphate Assay Kit
POMG-25H	BioAssay Systems	2500	EUR 330
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Malachite Green Phosphate Assay Kit
Z5030012	Biochain	2,500 assays	EUR 410.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Malachite Green Phosphate Assay kit
65-POMG-25H	Gentaur Genprice	2500 tests	EUR 333

HDAC Fluorogenic Assay Kit (Green)
50034	BPS Bioscience	96 rxns.	EUR 450
Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening_x000D_and profiling applications. The kit comes in a convenient 96-well format, with all the_x000D_reagents necessary for 100 fluorescent HDAC activity measurements. In_x000D_addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor,_x000D_Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC_x000D_Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_steps on a microtiter plate are needed to analyze the HDAC activity level. First,_x000D_the HDAC fluorometric substrate, containing an acetylated lysine side chain, is_x000D_incubated with purified HDAC enzyme. The deacetylation sensitizes the_x000D_substrate so subsequent treatment with the Lysine Developer produces a_x000D_fluorophore that can then be measured using a fluorescence reader at 485 nm_x000D_(excitation)/528 nm (emission)._x000D__x000D_Our other HDAC kit (#50033) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However, the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate

Cell Meter™ Live Cell Caspase 2 Binding Assay Kit *Green Fluorescence*
20111	AAT Bioquest	25 Tests	EUR 367.2

Cell Meter™ Live Cell Caspase 6 Binding Assay Kit *Green Fluorescence*
20113	AAT Bioquest	25 Tests	EUR 367.2

Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence*
20115	AAT Bioquest	25 Tests	EUR 367.2

Cell Meter™ Live Cell Caspase 9 Binding Assay Kit *Green Fluorescence*
20117	AAT Bioquest	25 Tests	EUR 367.2

Cell Meter™ Live Cell Caspase 10 Binding Assay Kit *Green Fluorescence*
20119	AAT Bioquest	25 Tests	EUR 367.2

Cell Meter™ Live Cell Caspase 13 Binding Assay Kit *Green Fluorescence*
20125	AAT Bioquest	25 Tests	EUR 367.2

Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence*
22796	AAT Bioquest	200 Tests	EUR 262.8

Caspase 12 Assay Kit
55R-1290	Fitzgerald	25 assays	EUR 393.6
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

Caspase 3 Assay Kit
20-abx294003	Abbexa	EUR 1506.00 EUR 548.40 EUR 924.00	100 tests 20 tests 50 tests

Poly-Caspase Assay Kit
20-abx299016	Abbexa	EUR 1562.40 EUR 698.40	100 tests 25 tests

Caspase 6 Assay Kit
20-abx299018	Abbexa	EUR 1562.40 EUR 698.40	100 tests 25 tests

Caspase 8 Assay Kit
20-abx299020	Abbexa	EUR 1562.40 EUR 698.40	100 tests 25 tests

Caspase 9 Assay Kit
20-abx299021	Abbexa	EUR 1562.40 EUR 698.40	100 tests 25 tests

Poly-Caspase Assay Kit
abx299022-2550tests	Abbexa	25-50 tests	EUR 830.4

Poly-Caspase Assay Kit
20-abx299025	Abbexa	EUR 1562.40 EUR 698.40	100 tests 25 tests

Caspase 2 Assay Kit
abx299026-25tests	Abbexa	25 tests	EUR 698.4

Caspase 10 Assay Kit
abx299027-100tests	Abbexa	100 tests	EUR 1562.4

Caspase 9 Assay Kit
20-abx299035	Abbexa	EUR 1562.40 EUR 698.40	100 tests 25 tests

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)
30011-1	Biotium	1KIT	EUR 244.8
Description: Minimum order quantity: 1 unit of 1KIT

Cell Meter™ Live Cell Caspase 3/7 Binding Assay Kit *Green Fluorescence*
20100	AAT Bioquest	25 Tests	EUR 367.2

EZCell? Phagocytosis Assay Kit (Green Zymosan)
K397-100	Biovision	each	EUR 516

Genorise® Caspase-1 Colorimetric Assay Kit
GR107007	Genorise Scientific	100 tests	EUR 456

Genorise® Caspase-1 Fluorometric Assay Kit
GR107008	Genorise Scientific	100 tests	EUR 420

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit
P-3001	EpiGentek	EUR 1050.06 EUR 424.60 EUR 712.80	96 Assays 48 Assays 96 Assays

EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K4)
P-3002	EpiGentek	EUR 1067.46 EUR 437.80 EUR 724.90	96 Assays 48 Assays 96 Assays

EpiQuik Histone Methyltransferase Activity/Inhibition Assay Kit (H3K9)
P-3003	EpiGentek	EUR 1067.46 EUR 437.80 EUR 724.90	96 Assays 48 Assays 96 Assays

Caspase 3 Fluorometric Assay Kit
55R-1269	Fitzgerald	25 assays	EUR 332.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 3 Colorimetric Assay Kit
55R-1270	Fitzgerald	25 assays	EUR 332.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 8 Fluorometric Assay Kit
55R-1274	Fitzgerald	25 assays	EUR 332.4
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit
55R-1275	Fitzgerald	25 assays	EUR 332.4
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Fluorometric Assay Kit
55R-1276	Fitzgerald	25 assays	EUR 344.4
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit
55R-1277	Fitzgerald	25 assays	EUR 340.8
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Fluorometric Assay Kit
55R-1278	Fitzgerald	25 assays	EUR 340.8
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit
55R-1279	Fitzgerald	25 assays	EUR 363.6
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 9 Fluorometric Assay Kit
55R-1280	Fitzgerald	25 assays	EUR 332.4
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 9 Colorimetric Assay Kit
55R-1281	Fitzgerald	25 assays	EUR 355.2
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 5 Fluorometric Assay Kit
55R-1282	Fitzgerald	25 assays	EUR 340.8
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit
55R-1283	Fitzgerald	25 assays	EUR 363.6
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Fluorometric Assay Kit
55R-1284	Fitzgerald	25 assays	EUR 340.8
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit
55R-1285	Fitzgerald	25 assays	EUR 363.6
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Fluorometric Assay Kit
55R-1286	Fitzgerald	25 assays	EUR 363.6
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit
55R-1287	Fitzgerald	25 assays	EUR 374.4
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 3/7 Assay Kit
20-abx299017	Abbexa	EUR 1562.40 EUR 698.40	100 tests 25 tests

Caspase 3/7 Assay Kit
abx299024-2550tests	Abbexa	25-50 tests	EUR 830.4

Caspase 3/7 Assay Kit
20-abx299028	Abbexa	EUR 1431.60 EUR 698.40	100 tests 25 tests

Caspase-2 Activity Assay Kit
AR4004-100Assays	BosterBio	100 Assays	EUR 616.8

Caspase-2 Activity Assay Kit
AR4004-25Assays	BosterBio	25 Assays	EUR 283.2

Caspase-2 Activity Assay Kit
AR4004-50Assays	BosterBio	50 Assays	EUR 408

QuantiFluo Caspase-3 Assay Kit
DCS3-100	BioAssay Systems	100	EUR 330
Description: Quantitative determination of the apoptosis target caspase-3 activity by fluorimetry (400/490nm) method. Procedure: 60 min. Kit size: 100 tests. Shelf life: 12 months.

Caspase-5 Fluorometric Assay Kit
K2195-100	ApexBio	100 assays	EUR 602.4

Caspase-5 Fluorometric Assay Kit
K2195-200	ApexBio	200 assays	EUR 835.2

Caspase-5 Fluorometric Assay Kit
K2195-25	ApexBio	25 assays	EUR 285.6

Caspase-5 Fluorometric Assay Kit
K2195-400	ApexBio	400 assays	EUR 1336.8

Caspase-5 Colorimetric Assay Kit
K2196-100	ApexBio	100 assays	EUR 634.8

Caspase-5 Colorimetric Assay Kit
K2196-200	ApexBio	200 assays	EUR 870

Caspase-5 Colorimetric Assay Kit
K2196-25	ApexBio	25 assays	EUR 301.2

Caspase-5 Colorimetric Assay Kit
K2196-400	ApexBio	400 assays	EUR 1387.2

Caspase-10 Colorimetric Assay Kit
K2197-100	ApexBio	100 assays	EUR 634.8

Caspase-10 Colorimetric Assay Kit
K2197-200	ApexBio	200 assays	EUR 885.6

Caspase-10 Colorimetric Assay Kit
K2197-25	ApexBio	25 assays	EUR 301.2

Caspase-10 Colorimetric Assay Kit
K2197-400	ApexBio	400 assays	EUR 1336.8

Caspase-4 Fluorometric Assay Kit
K2198-100	ApexBio	100 assays	EUR 634.8

Caspase-4 Fluorometric Assay Kit
K2198-200	ApexBio	200 assays	EUR 885.6

Caspase-4 Fluorometric Assay Kit
K2198-25	ApexBio	25 assays	EUR 301.2

Caspase-4 Fluorometric Assay Kit
K2198-400	ApexBio	400 assays	EUR 1336.8

Caspase-4 Colorimetric Assay Kit
K2199-100	ApexBio	100 assays	EUR 668.4

Caspase-4 Colorimetric Assay Kit
K2199-200	ApexBio	200 assays	EUR 885.6

Caspase-4 Colorimetric Assay Kit
K2199-25	ApexBio	25 assays	EUR 319.2

Caspase-4 Colorimetric Assay Kit
K2199-400	ApexBio	400 assays	EUR 1336.8

Caspase 9 Assay Kit, red
KF17302	Neuromics	25 Tests	EUR 364.8

Caspase-3 Fluorometric Assay Kit
K2007-200	ApexBio	200 assays	EUR 835.2

Caspase-3 Fluorometric Assay Kit
K2007-25	ApexBio	25 assays	EUR 285.6

Caspase-3 Fluorometric Assay Kit
K2007-400	ApexBio	400 assays	EUR 1303.2

Caspase-3 Colorimetric Assay Kit
K2008-200	ApexBio	200 assays	EUR 835.2

Caspase-3 Colorimetric Assay Kit
K2008-25	ApexBio	25 assays	EUR 285.6

Caspase-3 Colorimetric Assay Kit
K2008-400	ApexBio	400 assays	EUR 1303.2

Caspase-8 Fluorometric Assay Kit
K2012-200	ApexBio	200 assays	EUR 835.2

Caspase-8 Fluorometric Assay Kit
K2012-25	ApexBio	25 assays	EUR 285.6

Caspase-8 Fluorometric Assay Kit
K2012-400	ApexBio	400 assays	EUR 1303.2

Caspase-8 Colorimetric Assay Kit
K2013-200	ApexBio	200 assays	EUR 835.2

Caspase-8 Colorimetric Assay Kit
K2013-25	ApexBio	25 assays	EUR 285.6

Caspase-8 Colorimetric Assay Kit
K2013-400	ApexBio	400 assays	EUR 1303.2

Caspase-6 Fluorometric Assay Kit
K2014-200	ApexBio	200 assays	EUR 835.2

Caspase-6 Fluorometric Assay Kit
K2014-25	ApexBio	25 assays	EUR 285.6

Caspase-6 Fluorometric Assay Kit
K2014-400	ApexBio	400 assays	EUR 1303.2

Caspase-6 Colorimetric Assay Kit
K2015-200	ApexBio	200 assays	EUR 835.2

Caspase-6 Colorimetric Assay Kit
K2015-25	ApexBio	25 assays	EUR 285.6

Caspase-6 Colorimetric Assay Kit
K2015-400	ApexBio	400 assays	EUR 1321.2

Caspase-2 Fluorometric Assay Kit
K2016-200	ApexBio	200 assays	EUR 835.2

Caspase-2 Fluorometric Assay Kit
K2016-25	ApexBio	25 assays	EUR 285.6

Caspase-2 Fluorometric Assay Kit
K2016-400	ApexBio	400 assays	EUR 1336.8

Caspase-2 Colorimetric Assay Kit
K2017-200	ApexBio	200 assays	EUR 870

Caspase-2 Colorimetric Assay Kit
K2017-25	ApexBio	25 assays	EUR 301.2

Caspase-2 Colorimetric Assay Kit
K2017-400	ApexBio	400 assays	EUR 1387.2

Caspase-9 Fluorometric Assay Kit
K2018-200	ApexBio	200 assays	EUR 802.8

Caspase-9 Fluorometric Assay Kit
K2018-25	ApexBio	25 assays	EUR 285.6

Caspase-9 Fluorometric Assay Kit
K2018-400	ApexBio	400 assays	EUR 1336.8

Caspase-9 Colorimetric Assay Kit
K2019-200	ApexBio	200 assays	EUR 835.2

Caspase-9 Colorimetric Assay Kit
K2019-25	ApexBio	25 assays	EUR 301.2

Caspase-9 Colorimetric Assay Kit
K2019-400	ApexBio	400 assays	EUR 1387.2

Caspase-12 Fluorometric Assay Kit
K2150-100	ApexBio	100 assays	EUR 634.8

Caspase-12 Fluorometric Assay Kit
K2150-25	ApexBio	25 assays	EUR 301.2

Caspase-3 Fluorometric Assay Kit
K105-100	Biovision	each	EUR 574.8

Caspase-3 Fluorometric Assay Kit
K105-200	Biovision	each	EUR 744

Caspase-3 Fluorometric Assay Kit
K105-25	Biovision	each	EUR 248.4

Caspase-3 Fluorometric Assay Kit
K105-400	Biovision	each	EUR 1174.8

Caspase-3 Colorimetric Assay Kit
K106-100	Biovision	each	EUR 574.8

Caspase-3 Colorimetric Assay Kit
K106-200	Biovision	each	EUR 757.2

Caspase-3 Colorimetric Assay Kit
K106-25	Biovision	each	EUR 248.4

Caspase-3 Colorimetric Assay Kit
K106-400	Biovision	each	EUR 1149.6

Caspase-8 Fluorometric Assay Kit
K112-100	Biovision	each	EUR 574.8